An auto-stainer (AS), whole-slide imaging system (WSI), and computer
software were used as quality assurance tools for breast cancer immunohistochemistry
(IHC).
Cell lines, such as MCF-7 for estrogen receptors and progesterone receptors, and SKBR-
3 for Topo2a, were co-stained with samples as IHC control.
Combinatory usage of AS, WSI, computer software and cell lines can reduce deviation
in each IHC step and is beneficial for IHC quality assurance.
Keywords: Auto-analysis, Breast cancer, Immunohistochemistry, Quality
assurance, Whole slide image.