Stem cells are a category of cells with self-renewal and multi-lineage
differentiation capacity, which have been recognized as advantaged sources for tissue
engineering and regenerative medicine. To date, stem cells and their derivatives alone
or combined with biomaterials have aroused extensive and sustained attention to
investigations in the field of fundamental research and clinical practice. In recent years,
a series of novel technologies have been involved in stem cell-based cytotherapy, such
as three-dimensional (3D) printing, organoid research, and multitudinous kinds of
gene-editing technologies, which collectively facilitate the development of tissue
engineering for disease administration. In this chapter, we summarized the rudimentary
knowledge of the aforementioned new technologies, together with the promising
perspective and the concomitant challenges, which would help increase the cognition
of technological innovation for stem cell-based investigations and remedies in the
future.
Keywords: Bioprinting products, CRISPR/Cas9, CAR-T, Deoxyribonucleic acid, Gene knock-out, Gene knock-in, Human embryonic stem cells, Human induced pluripotent stem cells, In vivo organogenesis, Kidney organoid, Lipid nanoparticles, Modeling diseases, Nolecular mechanisms, Organoids, Single-cell transcriptomics, Stem cells, Scaffolds, TALENs, Three-dimensional printing, Tissue engineering.