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Anti-Infective Agents


ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

Research Article

Evaluation of In-Vitro Antioxidant Potential and Antimicrobial Activity of Nephrolepis biserrata (sw.) Schott. Leaf Extracts

Author(s): Sonia Singh*

Volume 20, Issue 4, 2022

Published on: 15 July, 2022

Article ID: e010522204320 Pages: 8

DOI: 10.2174/2211352520666220501163415

Price: $65


Objective: This study aims to determine the in vitro antioxidant and antimicrobial properties of Nephrolepis biserrata (Sw.) Schott leaf extracts against different microbial strains, including 4 gram-positive bacteria, 4 gram-negative bacteria, 3 yeast, and 4 mould.

Methods: The agar well diffusion method examined the antimicrobial activity of Nephrolepis biserrata leaf extracts against test micro-organisms. Additionally, TPC (total phenolic content), TFC (total flavonoid content), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay of extracts were determined.

Results: Preliminary phytochemical screening of all three extracts revealed the presence of tannins, terpenoids, alkaloids, saponin glycosides, and flavonoids. Results obtained were compared with the antibiotics Amphotericin B, Fluconazole, and Gentamicin used as standards. The mean zones of inhibition of methanol extract varied from 7 to 25 mm. However, with petroleum ether extract, the range varied from 9 to 12 mm and with acetone extract, the range was from 8 to 13 mm at two different concentrations of 250 and 500μg/disc. All extracts possessed significant antimicrobial activity against bacterial strains including Bacillus cereus, B. subtilis, Staphylococcus aureus, S. epidermis, Pseudomonas aeuginosa, Escherichia coli, Proteus Vulgaris, and Klebsiella pneumonia compared to fungal microbes such as Cryptococcus luteolus, Candida albicans, C. tropicalis, Aspergillus candidus, A. niger, and Mucor hiemalis wehmer, respectively. However, amongst all the three extracts, methanol leaf extract showed maximum antimicrobial activity.

Conclusion: From the present study, it has been summarized that the antimicrobial activity of plants might be due to the presence of flavonoids and tannin components. In conclusion, still advanced research is required to isolate the active principles from plant extracts, showing antimicrobial activity that may lead to the development of a phytomedicine.

Keywords: Antimicrobial, methanol, microbial strains, phytomedicine, zone of inhibition, amphotericin b, fluconazole.

Graphical Abstract
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