Applications of Modern Mass Spectrometry

Volume: 2

LC-MS Analysis of Endogenous Neuropeptides from Tissues of Central Nervous System: An Overview

Author(s): Neva Alasağ* and Erol Şener

Pp: 1-61 (61)

DOI: 10.2174/9789815050059122020003

* (Excluding Mailing and Handling)


In recent years, various methods and technological advances demonstrated
that neurochemical measurements have contributed to significant improvements in our
understanding of the relationship between chemistry in the central nervous system
(CNS) and the organism. Techniques based on Liquid Chromatography-Mass
Spectrometry (LC-MS) are potent approaches for separating and quantifying
endogenous neuropeptides in CNS. The separation ability and reliability of LC with
sensitivity and selectivity of MS have become a valuable combination for peptide
analysis either qualitatively or quantitively. Thus, new peptides have been identified
using this technique. When applied to disease models, pathophysiological mechanisms
can be identified and used as drug targets or biomarkers. Due to the low concentrations
of neuropeptides in the biological samples, they restrict developing analysis methods
and the understanding of their biological function. This book chapter focuses on novel
developments of LC-MS/MS for endogenous neuropeptides. It has also emphasized the
applications that cite preparation techniques used for brain tissue analysis, published in
recent years.

Keywords: LC-MS/MS, Endogenous neuropeptide, Sample preparation, Brain tissues, Microdialysis, Central nervous system, Neuropeptide families, Mass spectrometry, Mass analyzers, Ionization sources, Fragmentation techniques, Neuropeptide analysis.

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