Ion Mobility-Mass Spectrometry for Macromolecule Analysis

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* (Excluding Mailing and Handling)

The need for conformational information is increasing by the time in studies

on macromolecules. For example, proteins may have various functions and properties

depending on their folding states that make their conformational analyses very

important. Mass spectrometry is one of the most effective analytical techniques that

separate ions in the gas phase by their mass-to-charge ratio. It provides useful data on

molecular characterization in many areas of research with high precision, accuracy, and

sensitivity. Although mass spectrometry is a very powerful analytical technique, it

cannot distinguish different species having identical mass-to-charge ratio. The

analytical technique combining mass spectrometry with ion mobility spectrometry

(IM-MS), which provides information about the three-dimensional structure of an ion,

solves this problem by separating them according to their collision cross sections

(CCS) in the gas phase. This analytical method also provides the advantages of higher

precision and better resolution in the rapid analysis of different types of complex

samples. The separation of isomers with the same molecular weight, increasing the

dynamic range and distinguishing ions from chemical noise are the most important

features that this technique contributes to mass spectrometry. As improvements have

been made in IM-MS technology, the number and quality of publications in the areas

where this technique is used increases rapidly. In this chapter, the use of IM-MS

techniques in the fields such as proteomics, glycoproteomics and polymer

characterization are explained by presenting their various applications in the literature.

Keywords: Conformational Characterization, Glycoproteomics, Ion Mobility- Mass Spectrometry, Proteomics, Polymer Characterization.

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Current Chinese Science

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DOI https://doi.org/10.2174/9789815050059122020001	Print ISSN 2717-6029
Publisher Name Bentham Science Publisher	Online ISSN 2717-6037

Applications of Modern Mass Spectrometry