The use of fluorescence microscopy requires knowledge of the elements
making up the fluorescence microscope. In this chapter the basic elements combining
to make a fluorescence microscope will be introduced and its role explained.
Afterwards an introduction to the most common fluorescence microscopic techniques
will be made followed by more advanced and developed approaches. These will
include total-internal reflection and time-resolved microscopies, to name a few. The
chapter will end with a brief introduction to flow cytometry, a highly related
fluorescence technique in the biomedical field.
Keywords: Confocal microscope, Filter, Flow cytometry, Fluorescence
Correlation Spectroscopy (FCS), Fluorescence Lifetime Imaging Microscopy
(FLIM), Fluorescence localization after photobleaching (FLAP), Fluorescence
loss in photobleaching (FLIP), Fluorescence microscope, Fluorescence
photobleaching, Fluorescence recovery after photobleaching (FRAP), Imaging
system, Light-sheet microscope, Microscope objective, Time-resolved
microscopy, Total Internal Reflection Fluorescence Microscopy (TIRFM).
