Title:Identification of Key mRNAs, miRNAs, and mRNA-miRNA Network Involved in Papillary Thyroid Carcinoma
Volume: 16
Issue: 1
Author(s): Wei Han*, Dongchen Lu*, Chonggao Wang, Mengdi Cui and Kai Lu
Affiliation:
- Department of Thyroid and Breast Surgery, Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210000,China
- Department of Thyroid and Breast Surgery, Nanjing Hospital of Chinese Medicine affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210000,China
Keywords:
microRNA, papillary thyroid carcinoma, network, pathogenesis, gene, bioinformatics.
Abstract: Background: In the past decades, the incidence of thyroid cancer (TC) has been gradually
increasing, owing to the widespread use of ultrasound scanning devices. However, the key mRNAs,
miRNAs, and mRNA-miRNA network in papillary thyroid carcinoma (PTC) has not been fully
understood.
Methods: In this study, multiple bioinformatics methods were employed, including differential
expression analysis, gene set enrichment analysis, and miRNA-mRNA interaction network
construction.
Results: Firstly, we investigated the key miRNAs that regulated significantly more differentially
expressed genes based on GSEA method. Secondly, we searched for the key miRNAs based on the
mRNA-miRNA interaction subnetwork involved in PTC. We identified hsa-mir-1275, hsa-mir-1291,
hsa-mir-206 and hsa-mir-375 as the key miRNAs involved in PTC pathogenesis.
Conclusion: The integrated analysis of the gene and miRNA expression data not only identified key
mRNAs, miRNAs, and mRNA-miRNA network involved in papillary thyroid carcinoma, but also
improved our understanding of the pathogenesis of PTC.