This book is a comprehensive review of secondary metabolite production from plant tissue culture. The editors have compiled 12 meticulously organized chapters that provide the relevant theoretical and practical frameworks in this subject using empirical research findings. The goal of the book is to explain the rationale behind in vitro production of secondary metabolites from some important medicinal plants. Biotechnological strategies like metabolic engineering and the biosynthesis, transport and modulation of important secondary metabolites are explained along with research studies on specific plants. In addition to the benefits of secondary metabolites, the book also aims to highlight the commercial value of medicinal plants for pharmaceutical and healthcare ventures. Topics covered in this part include: 1. In vitro propagation and tissue culture for several plants including Withania somnifera (L.) Dunal, Aloe vera, Oroxylum indicum (L) Kurz, Ocimum basilicum L, Rhubarb, Tea, and many others (including plants in Northern India). 2. Genetic Improvement of Pelargonium 3. Bioactive Components in Senna alata L. Roxb 4. Plant tissue culture techniques The book caters to a wide readership. It primarily prepares graduate students, researchers, biotechnologists, giving them a grasp of the key methodologies in the secondary metabolite production. It is a secondary reference for support executives, industry professionals, and policymakers at corporate and government levels to understand the importance of plant tissue culture and maximizing its impact in the herbal industry.

Aromatherapy is a medical practice that uses aromatic compounds or essential oils to influence mood and health. Essential oils used in aromatherapy are created from a wide variety of medicinal plants, flowers, herbs, roots, and trees that are found all over the world and have significant, well-documented benefits on enhancing physical, emotional, and spiritual wellbeing. This book is a comprehensive reference on aromatic compounds present in essential oils and their therapeutic use. Starting from fundamentals of essential oil biosynthesis the book guides the reader through their basic biochemistry, toxicology, profiling, blending and clinical applications. The concluding chapters also present focused information about the therapeutic effects of essential oils on specific physiological systems, plant sources, skin treatment and cancer therapeutics. The combination of basic and applied knowledge will provide readers with all the necessary information for understanding how to develop preclinical formulations and standard clinical therapies with essential oils. This is an essential reference for anyone interested in aromatherapy and the science of essential oils.

This reference is a comprehensive review of genome editing in bacteria. The multi-part book meticulously consolidates research findings and insights on the applications of bacteria across several industries, including food processing and pharmaceutical development. The book covers four overarching themes for readers: a historical perspective of genome editing, genome editing in probiotics, applications of genome editing in agricultural microbiology and genetic engineering in environmental microbiology. The editors have also compiled chapters that provide an in-depth analysis of gene regulation and metabolic engineering through genome editing tools for specific bacteria. Key topics in part 2: - Targeting pathogenic microbes for plants and animals using CRISPR-CAS - Genome editing microbes to improve crop yield plant growth for sustainable agriculture - Applications of genome editing for bioremediation - Microbial genome editing for environmental bioprocessing - Genetic engineering for methanotrophs - Genome engineering in Cyanobacteria - Genome editing in Streptomyces Genome Editing in Bacteria is a definitive reference for scholars, researchers and industry professionals navigating the forefront of bacterial genomics.

PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors. This E-book discusses the basic concepts, chemistries, and instrumentation of standard and real-time PCR, and includes present applications and future perspectives for veterinary diagnostics. Critical pitfalls have been highlighted in this text with regard to veterinary PCR diagnostics which include: i) choice of platform technologies for PCR diagnosis; ii) construction of optimized PCR primers and probes to ensure its highest specificity and sensitivity; iii) correct sampling and efficient methods to preserve and release nucleic acids; iv) maximization of PCR amplification efficiency; and v) avoidance and monitoring of contamination in Veterinary PCR Diagnostics. This eBook should be a valuable reference for all veterinary professionals interested in this modern diagnostic tool.