摘要
心肌细胞损伤及其后遗症是全球死亡的主要原因。不幸的是,成人心肌细胞的再生能力有限。因此,从非心肌细胞中生成新心肌细胞是一种替代策略。转录因子(TFs)可以被招募来实现这一巨大的目标。转录组分析表明,GATA、Mef2c和Tbx5 (GMT鸡尾酒)是将成纤维细胞、体细胞、中胚层细胞转分化/重编程为心肌细胞的主转录因子。然而,添加MESP1、心肌、ESRRG和ZFPM2 TFs诱导诱导心肌细胞产生更高效的生理特征。此外,同样的转录因子鸡尾酒可以诱导诱导/多能干细胞向心肌细胞的增殖和分化。受损心肌细胞的改善包括炎症介质诱导的愈合转录因子的激活;IL6、肿瘤生长因子β和IL22。转录因子调节心肌细胞的细胞和亚细胞生理,包括有丝分裂细胞周期调节、核分裂和胞质分裂、增生性生长、成体肌收缩蛋白基因表达、脂肪酸代谢和线粒体生物发生和成熟。转录因子细胞治疗可用于心脏发生和改善受损心肌细胞。转录因子是细胞分化的基础。
关键词: 心肌梗死,再生,心肌细胞,转录因子,转分化,重编程,血管新生,细胞谱系,重编程,细胞可塑性,体细胞,干细胞。
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