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The Natural Products Journal


ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Research Article

In Vitro Antioxidant and Anti-Convulsant Activity of Methanolic Extract of Sarasvata churna Using scPTZ and MES Models

Author(s): Rahul Kaushik*, Jainendra Jain and Avijit Mazumder

Volume 11, Issue 2, 2021

Published on: 17 January, 2020

Page: [244 - 255] Pages: 12

DOI: 10.2174/2210315510666200117151832

Price: $65


Background: Sarasvata churna is an Ayurvedic formulation for treatment and management of epilepsy and other maniac disorders since thousands of years.

Objective: This study aimed to evaluate the in vitro antioxidant potential, total phenolics and flavonoids content, acute-oral-toxicity and anticonvulsant activity of Sarasvata churna.

Materials & Methods: In vitro antioxidant activity of methanolic extract of Sarasvata churna against Nitrous oxide, Peroxide, Phosphomolybdenum and Hydroxyl radicals was performed using Colorimetry against Ascorbic acid as standard along with estimation of total phenolic and flavonoids content. Acute oral toxicity was evaluated using OECD guidelines. Extract in carboxymethyl cellulose at doses of 50,75,100,125,150 and 200 mg/kg was screened for anticonvulsant activity using subcutaneous Pentylenetetrazole and Maximal Electroshock models in Swiss Albino Mice (n=6). Sodium valproate was used as standard.

Results: IC50 value of methanolic extract in the Nitrous oxide, Peroxide, and Hydroxyl free radical scavenging assay was found to be 165mg/ml, 32.5mg/ml and 253.9mg/ml respectively as compared to 61.58μg/ml, 333.44μg/ml and 351μg/ml respectively of standard Ascorbic acid. In acute oral toxicity screening, animals did not show any signs of acute and delayed toxicity even up to a dose of 2000mg/kg. Extract offered a protection of 57.39% and 85.26% in scPTZ model (P<0.0001) and 74% and 96.38% in MES model (P<0.0001) at doses of 50 and 200mg/kg respectively as compared to standard at 95% Confidence interval (ANNOVA, Tukey test) indicating a dose-dependent protection.

Conclusion: Sarasvata churna’s potentials are comparable with standard antioxidant Ascorbic acid and antiepileptic drug Sodium valproate. This preclinical and toxicity screening data can be beneficial in establishing the scientific basis for the use of Sarasvata churna in management of epilepsy.

Keywords: Anticonvulsant, Sarasvata churna, polyherbal, ayurvedic, epilepsy, ANNOVA.

Graphical Abstract
Trinka, E.; Cock, H.; Hesdorffer, D.; Rossetti, A.O.; Scheffer, I.E.; Shinnar, S.; Shorvon, S.; Lowenstein, D.H. A definition and classification of status epilepticus - Report of the ILAE task force on classification of status epilepticus. Epilepsia, 2015, 56(10), 1515-1523.
Goldenberg, M.M. Overview of drugs used for epilepsy and seizures: etiology, diagnosis, and treatment. Pharm. Ther., 2010, 35(7), 392-415.
Aneja, S.; Sharma, S. Newer anti-epileptic drugs. Indian Pediatr., 2013, 50(11), 1033-1040.
Kaushik, R.; Jain, J.; Mazumdar, A.; Singh, L. Studying the pharmacological basis of an antiepileptic Ayurvedic formulation- Sarasvata Churna. Int. J. Green Pharm., 2017, 11(2), 1-7.
Ayurvedic Formulary of India. National Institute of Science Communication, CSIR. Part-II, First English Edition; Ayurvedic Formulary of India: New Delhi, 2000, pp. 97-98.
Kaushik, R.; Jain, J.; Mazumder, A. Chromatographic fingerprinting of sarasvata churna-an ayurvedic polyherbal formulation for epilepsy. J. Appl. Pharm. Sci., 2018, 8(4), 090-098.
Marcocci, L.; Maguire, J.J.; Droy-Lefaix, M.T.; Packer, L. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem. Biophys. Res. Commun., 1994, 201(2), 748-755.
Govindarajan, R.; Rastogi, S.; Vijayakumar, M.; Shirwaikar, A.; Rawat, A.K.; Mehrotra, S.; Pushpangadan, P. Studies on the antioxidant activities of Desmodium gangeticum. Biol. Pharm. Bull., 2003, 26(10), 1424-1427.
Keser, S.; Celik, S.; Turkoglu, S.; Yilmaz, O.; Turkoglu, I. Hydro-gen peroxide radical scavenging and total antioxidant activity of hawthorn. Chem. J., 2012, 2(1), 9-12.
Nabavi, S.M.; Ebrahimzadeh, M.A.; Nabavi, S.F.; Hamidinia, A.; Bekhradnia, A.R. Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey. Pharmacol. Online, 2008, 2(53), 560-567.
Halliwell, B.; Gutteridge, J.M. Formation of thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Lett., 1981, 128(2), 347-352.
Singleton, V.L.; Rossi, J.A., Jr Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 1965, 16(3), 144-158.
Prieto, P.; Pineda, M.; Aguilar, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem., 1999, 269(2), 337-341.
Singleton, V.L.; Orthofer, R.; Lamuela-Raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol., 1999, 299, 152-178.
OECD 420 Guidelines for testing of chemicals, adopted on 17 Jan., 2001. Available from:
Giramkar, S.A.; Kulkarni, O.S.; Jagtap, S.D.; Kuvalekar, A.; Mukherjee, S.; Jagtap, R.R.; Wagh, A.R.; Bandawane, D.D.; Nipate, S.S. Anticonvulsant potential of commonly practiced formulations of Brahmi (Bacopa monnieri Linn.) in Wistar rats. J. Pharm. Res., 2013, 7(9), 787-791.
Ghosh, M.N. Fundamentals of Experimental Pharmacology, 5th ed; Hilton and Company: United Kingdom, 2011.
Achliya, G.S.; Wadodkar, S.G.; Dorle, A.K. Evaluation of CNS activity of Brahmi Ghrita. Int. J. Pharmacol., 2005, 37(1), 33-36.
Majumdar, R.; Bagade, A. Seizure control and pattern of behavior in children with simple febrile convulsions- Effect of Mentat syrup. In Pract., 2001, 54(10), 729-733.
Snead, O.C., III Pharmacological models of generalized absence seizures in rodents. J. Neural Transm. Suppl., 1992, 35, 7-19.
Swinyard, E.A.; Kupterberg, H.J. Antiepileptic drugs: detection, quantification and evaluation. Fed. Proc., 1985, 44(10), 629-630.

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