The present chapter highlights the various newer and advanced real-time
quantitative PCR (qPCR) detection chemistries and their applications in biomedical
research and molecular diagnostics. The chapter also summarizes the most advanced
modification of the qPCR based technique along with next generation methods which
have immense potential to revolutionize the field of biomedical sciences and molecular
diagnostics.
Keywords: Molecular diagnostics, qPCR, SYBR green.