Animal cell culture techniques in today’s scenario have become an
indispensable tool in the field of biomedical research. It provides a basis to study
molecular and biochemical changes associated with disease pathogenesis. It explicitly
provides a scope to study gene expressions, regulation, proliferation, and differentiation
in normal as well as pathologic conditions. The culturing of animal cells requires
aseptic conditions and vital technical skills to carry out successful cell culture
experiments. It provides an appropriate model for studying cell and molecular biology,
biochemical changes in cells, drug screening and efficacy etc. This chapter describes
the essential techniques of animal cell culture as well as its applications.
Keywords: Animal cell culture, Adherent cells, Bacterial contamination,
Biomedical research, Biomedical applications, Cell counting, Cell lines, Cell
viability, Contaminants, Cross-contamination, Cryopreservation, Laboratory
instruments, Mycoplasma contamination, MTT assay, Primary cell cultures,
Secondary cell cultures, Suspension cells, Thawing of cells, Virus contamination,
Yeast contamination.