Immunohistochemical (IHC) and molecular findings with their
histopathological diagnosis are critical in the current therapeutic strategy for a breast
cancer patient. These findings require considerable manual human involvement and
interpretations can be subjective. In this study, we examined the usefulness of an autoanalysis
computer system for analysis of IHC findings.
Forty breast-cancer specimens were examined for IHC expressions of estrogen receptor
(ER), progesterone receptor (PgR), HER2, Ki-67 and Topoisomerase II alpha
(TopoIIa). One-hundred-fifty-six cancer specimens were examined for HER2.
Histopathological images of IHC specimens were stored digitally thru virtual
microscopy (Hamamatsu Photonics). A Genie/Aperio software package on a desktop
computer auto-analyzed these images.
For ER and PgR, concordant results were obtained between ocular observation and
auto-analysis (p < 0.001). The Ki-67 index (r = 0.96) and TopoIIa index (r = 0.95) also
showed a significant correlation (p < 0.001). Concordant ratios were 92.9%, 82.2%,
59.5% and 100% for HER2 score 0, 1, 2 and 3, respectively. 24 cases (28.6%) of 84
cases with a HER2 score of 2 by ocular observation were re-analyzed as a score of 3 by auto-analysis. 19 cases (79.2%) of these re-scored cases showed HER2 gene
amplification by FISH analysis.
In sum, well-organized auto-analysis is able to provide objective results. Thus, autoanalysis
might be a means by which to standardize methods for immunohistochemical
detection of breast cancer.
Keywords: Auto analysis, Breast cancer, Immunohistochemistry, Ocular
observation, Virtual microscopy.
