Methods to Determine Enzymatic Activity

Qualitative and Quantitative to Determine Peptidase Activity

Author(s): Alane Beatriz Vermelho, Edilma Paraguai de Souza, Daniel Paiva and Giseli Capaci Rodrigues

Pp: 262-280 (19)

DOI: 10.2174/9781608053001113010015

* (Excluding Mailing and Handling)


The aim of this chapter is to present the modern analytical methodologies used to study peptidases, such as the colorimetric methods and spectrophotometric analyses. A general method for the detection of peptidases activity includes gelatin and other substrates incorporated into SDS-PAGE using a technique known as zymography. All methodologies presented here are important tools for the discovery and characterization of peptidases.

Keywords: Peptidase, protease, gelatin, metallopeptidases, isopropanol, zymography.

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