The lightning progress in the understanding of enzymatic mechanisms,
democratization of molecular biology tools and their introduction in chemistry
laboratories, the pressure of green chemistry notions leading to the need for alternative
sustainable and more environmentally friendly approaches, and probably economic
factors as well, all these parameters allowed the development of new synthetic
strategies based on biocatalysis. This chapter is dedicated to the use of glucosyl
hydrolases and some of their mutants, i.e., glucosynthases and thioligases, to the
preparation of β-(1,3)-(thio)glucosides.
Keywords: Glucan, thio, oligo, synthesis, donor, residue, biocatalysis, conjugate,
branching, laminarin, residue, biocatalyst, thioglycosides, strategy, carbohydrate,
NMR, glycans, transferase, enzymes, laminarin.
