Nanoparticles are being incorporated into many products of daily use, e.g. fillers, pacifiers,
catalysts, pharmaceuticals, lubricants, cosmetics, pharmaceuticals, electronic devices or other domestic
appliances. These approaches demonstrate the high potential of nanocomposites in cellular biotechnology as
well as for the development of more efficient methods to introduce foreign DNA to sperm cells and
consequently improve the techniques for transgenic animal technology. This chapter will address the main
approaches for the use of nanocomposites for gene delivery and the potential uses in nanoparticle spermmediated
gene transfer (Nano-SMGT).
Keywords: Nanostructures, Nanotubes, Nanopolymers, Nanoparticles, SMGT, Gene therapy, DNA/RNA
delivery, Sperm, Transfection, Transgenesis, Knockdown.
