This chapter discusses the key factors influencing liposome formation,
including size, stability, lamellarity, and encapsulation efficiency, and highlights their
importance across diverse applications. It investigates cutting-edge technologies, such
as TEM, SEM, and AFM, for assessing the size, shape, and structure of liposomes. The
chapter also discusses spectroscopic methods such as NMR and UV/Vis for analyzing
liposomes’ composition and behaviour. Furthermore, DLS is described as a quick
approach for determining size and stability, with a focus on optimizing its application
in liposome characterization. These technologies give critical insights for optimizing
liposomal formulations in a wide range of medicinal applications.
Keywords: Liposome formation, encapsulation efficiency, microscopy techniques, spectroscopy techniques, dynamic light scattering.
