This chapter discusses lipid hydrolysis and oxidation, which are critical
for liposome stability. Liposomes are vulnerable to these processes due to their lipidbased nature, which might compromise their structural soundness and functioning.
Fatty acids and LPCs are produced during hydrolysis, which destabilizes liposomes.
Enzymatic hydrolysis by lipases speeds up this breakdown even more, especially in
inflammatory environments. Conversely, oxidation results in hazardous compounds
that harm bilayer membranes. These processes, which are affected by temperature, pH,
and metal ions, can be stopped by antioxidants or proper storage. This chapter also
covers strategies for minimizing degradation in stable liposomal formulations and the
effects of pH, temperature, and buffer concentration on alkaline hydrolysis.
Keywords: Lipid degradation, oxidation, hydrolysis, liposome stability, antioxidants.
