Lipid extraction and fractionation are essential methods for understanding varied and complex composition of natural lipids. This chapter emphasizes the
need for the precise separation procedures for isolating and analyzing distinct lipid
classes. Column chromatography, thin-layer chromatography, and high-performance
liquid chromatography are investigated, emphasizing their concepts, advances, and
practical applications. Modern technologies, such as solid-phase extraction and chromatographic procedures combined with modern detection systems such as liquid
chromatography-mass spectrometry, are emphasized for their efficiency and high
resolution. These approaches are beneficial for isolating and characterizing specific
lipids required to form stable and functional liposomal structures, hence facilitating
the development of optimized formulations for liposome-based applications.
Keywords: Lipid extraction, fractionation, thin-layer chromatography, highperformance liquid chromatography, solid-phase extraction, lipid analysis.
