This chapter thoroughly reviews lipid extraction methods, emphasizing
their fundamentals, developments, and real-world uses. With an emphasis on effectiveness, sustainability, and safety, it examines established procedures like the Bligh and
Dyer and Folch processes and cutting-edge strategies like solid-phase extraction and
supercritical fluid extraction. In order to guarantee high-quality extraction for subsequent studies, the chapter covers important factors such as solvent selection, sample
preparation, and lipid preservation. It provides insights into contemporary lipidomics
by describing cutting-edge approaches like the Matyash and BUME (butanol-methanol
extraction) methodologies, which balance cost-effectiveness, environmental impact,
and application across various lipid classes.
Keywords: Lipid extraction, Bligh and Dyer, Folch method, Soxhlet extraction, green solvents.
