In the field of nematology, a variety of microscopes are utilized for distinct
purposes, including standard light microscopes and stereomicroscopes. Additionally,
line illustrations are essential for the taxonomical examination of nematodes. As such,
it is crucial to properly calibrate and maintain microscopes to ensure accurate
outcomes. This section delves into the essential duties associated with microscope
usage in nematological research. Differential interference contrast microscopy (DIC) is
a useful technique to create a high-quality photograph of the nematodes. In regard to
taxonomy and diagnosis of nematodes, accurate measurements are of utmost
importance. Each nematode family, whether they are free-living or plant-parasitic,
necessitates specific measurements of particular attributes. The identification of
nematodes relies on the de Man indices. This section offers visual aids for different
families to showcase the significant features that must be measured. The art of
capturing images of nematodes holds immense significance in the fields of taxonomy
and histological research. Nematology employs numerous methods to obtain top-notch
visuals for analysis, among which is Scanning Electron Microscopy (SEM).
Furthermore, phase contrast is widely used in nematology for a variety of purposes.
This chapter endeavors to provide a comprehensive understanding of the microscope,
measurements, DIC, and SEM techniques.
Keywords: Calibration, DIC, Microscope, SEM.
