Methods and Techniques in Nematology

Nematode Observations

Author(s): Ebrahim Shokoohi *

Pp: 29-35 (7)

DOI: 10.2174/9789815313680125010008

* (Excluding Mailing and Handling)

Abstract

Preservation of nematodes is a crucial element of research endeavors, and it can be accomplished either temporarily or permanently. In order to conduct a rapid examination, researchers may place a minute droplet of water onto a glass slide for temporary observation. However, for the purpose of long-term preservation and collection, permanent slides are requisite. Prior to studying nematodes under a microscope for taxonomic purposes, it is necessary to first kill and fix them. This method is particularly useful for taxonomic studies involving light, electron (SEM), or transmission electron (TEM) microscopy.


Keywords: Fixative, Glass slide, Permanent, Preserving, Temporarily.