S-nitrosylation, is a PTM (post translational modification of NO (nitric oxide). Abiotic stress conditions lead to NO evolution and SNO (s-nitosothiols, the cellular NO pool) accumulation. Recently, research on NO and nitrosylation with respect to stress has gained momentum. Partial S-nirosoproteome of Arabidopsis, K. pinata and B.juncea are identified following BST, affinity purification and mass spectometric identification. In this chapter a snapshot of the relevance of NO and nitrosylation in abiotic stress is presented.