A biosensor is a device that converts a biological response into a detectable
electrical signal. In recent years, biosensors have gained significant interest due to a
plethora of applications in the field of disease diagnosis, detection of various
environmental pollutants, food quality analysis, and pharmaceutical drug research.
Among various types of biosensors, (such as enzyme-based, immunosensors, DNA
biosensors, thermal and piezoelectric biosensors) DNA biosensors are being widely
employed because of superior biocompatibility, thermal stability and alternative
functionalization. DNA biosensors introduced in recent years include an aptamer-based
sensor, molecular beacon-based biosensors, fluorescence-based sensors,
hybridizationbased sensors and electrochemical-based DNA biosensors. This chapter
highlights the fundamental knowledge and recent advances in the field of DNA-based
biosensors. This chapter also focuses on the significance and wide application of DNAbased biosensors in the diverse areas of biotechnology and allied fields.
Keywords: Aptamers, Biosensor, Diagnosis, DNA-based, Environment, Fluorescence-based.