Agro-industrial waste production represents an environmental problem, and
its processing results in the obtainment of by-products that are rich sources of bioactive
compounds. Apis mellifera are social insects that create the ideal conditions for the
transmission of pathogens. American foulbrood (AFB) is the main pathology that
affects bee brood; the causative agent is the Gram-positive bacteria Paenibacillus
larvae. During the last years, there have been significant losses of hives by the use of
synthetic antimicrobials for the control of AFB. The presence of antibiotics in honey
and other products of the hive have generated concern in consumers regarding the risks
of toxicity, negatively influencing the time of marketing and export. On the other hand,
there is the possibility of the appearance of resistance by pathogenic microorganisms
caused by incomplete treatments or an overdose of the antibiotic used. More than 70
plant extracts have been evaluated in vitro and in vivo against P. larvae; for this reason,
the revaluation of biological waste material from agroindustry is being studied through
pharmacodynamic and pharmacognosy analysis both in P. larvae and in the different
stages of development of A. mellifera.
Therefore, this chapter proposes to investigate: (1) the effect of the application of
fertilizer treatment on the hop cultivar in the composition and amount of secondary metabolites, (2) the bioactivity of extracts of hop leaves against P. larvae, and in vitro
toxicity in larvae and adult bees, and the effect on genes of the immune system of bees.
Keywords: Agro-Industrial Waste, American Foulbrood, Antimicrobial Activity,
Apis Mellifera, Flavonoids, Honey Bee, Humulus lupulus, Paenibacillus Larvae,
Phenolic Compounds, Saponins, Secondary Metabolites.