Canine Circovirus (CaCV) or Dog Circovirus (DogCV) causes hemorrhagic
gastroenteritis, thrombocytopenia, vasculitis, hemorrhages, and neutropenia and is
usually found as co-infection with other canine infectious agents like Parvovirus and
Distemper virus in dogs including wild canines (wolf, fox, badger). To know the
evolutionary feature of the viruses, nucleotide composition and Codon usage bias
(CUB) have been used which further ascertain their adaptability towards the suitable
host. In the present study CUB and Nucleotide composition of both cap & rep gene of
around 88 CaCV strains were compared. Nucleotide composition of CDSs at 3rd codon
position (G3% A3% T3% C3%) together with overall AT% and GC%, GC12, and GC3
were analyzed. using codon W 1.4.2 and CAIcal server, Aromo, and Gravy values,
effective no of codons and relative synonymous codon usage values were also
analyzed. The results show that CaCV has an AT-rich genome and codons ending with
A/T have to preference over GC ending codons. Nc-GC3 plot of CanineCV reveals that
selection pressure was dominant over mutation pressure. Correlation analysis between
Gravy, Aroma, and CAI indicate natural selection over mutational pressure. The RSCU
values of Cap & Rep genes were analyzed to find out overrepresented codons.
Keywords: CaCV, Canine Circovirus, Codon usage bias, Dog Circovirus, RSCU.
