Neutralizing antibodies (nAbs) make a defense line against viral attacks by
binding to viruses to shield infections. A neutralizing antibody interferes with the virus
in different ways as it may block the cell receptor or bind to viral capsid by inhibiting
genome un-coating. The micro-neutralization (MN) assay is a basic technique for
detecting viruses in epidemiological, immunological, virological studies, and vaccine
assessment tests. The underlying mechanism of this assay is based on the detection of
specific antigen-antibody reactions. It only detects the antibodies involved in the
blockage of the virus replication. This technique is specifically helpful in evaluating
specific micro-organism serotype neutralizing antibodies in the sera of humans and
animals. It describes the neutralization or inhibition of replicating virus strains by
antibodies in the sera of humans and animals. But highly constraint provisions may be
required while working with live virus-based micro-neutralization techniques,
particularly when handling precarious micro-organisms like severe acute respiratory
syndrome-coronavirus-2 (SARS-CoV-2). Herein, all the possible data regarding
isolation, amplification, and titration of SARS-CoV-2 and MN assay to measure nAbs
level in the sera of mammalian species have been summarized.
Keywords: Antibodies, Microneutralization, Neutralization, SARS-CoV-2.
