Bacterial Biofilms are densely packed microbial communities formed by the
microorganism to escape from any external threat. These Biofilms are composed of a
polysaccharidic matrix, which formed a slimy layer outside the cell wall and protected
the microbes from any damage (both physical and chemical). Biofilm can be developed
by microorganisms on any surface, including medical devices, oral cavity and other
biomaterials. These biofilms are difficult to treat and required almost 10,000 more
concentrations of antibiotics compared to planktonic microorganisms. These biofilms
are the main hindrance in the treatment of hospital-acquired infections. Majority of the
treatment against microorganisms fails due to these biofilms in a clinical setting.
Combination of different antibiotics, natural molecules and other strategies is in use to
combat these biofilms. Microorganisms inside the Biofilm trigger some genes by
quorum sensing and affect the expression of several protein factors. The current chapter
will focus on the use of the proteomic approach for better understanding the nature and
role of Biofilm in microbial pathogenesis and lower the emergence of drug resistance
in these microorganisms.
Keywords: Antibiotics, Biofilm, Drug resistance, Microorganism, Pathogenesis,
Polysaccharidicmatrix, Proteomics.