Origanum majorana Linn. (Majorana hortensis) is an aromatic herb of Lamiaceae. The plant is native to Mediterranean and European parts, but can be cultivated easily in all tropical regions. The leaves and flowers are characterized by a pleasant aromatic odour that increases its scope for perfumery and food industries. Besides its culinary & perfumery importance, O. majorana has therapeutic relevance in the management of diabetes, hypertension, polycystic ovarian syndrome (PCOS), gastric ulcers, leukemia, breast adenocarcinoma, free radical scavenging, etc. The proposed chapter focuses on traditional uses, culinary and perfumery applications, recent advancements in phytochemistry and pharmacotherapeutics of Origanum majorana.