Raman spectroscopy is a significant characterization technique using inelastic scattering of light associated with molecular vibration to gather information about chemical fingerprints of tissues, cells or biofluids. Lack of sample preparation including chemical specificity and the ability to use advanced techniques in the visible or near-infrared spectral range have led versatility in biological applications of Raman spectroscopy. By Raman spectroscopy, the changes caused by diseases in tissues and organs can be accurately investigated and it is fast, non-invasive, economic and highly specific in comparison to other diagnostic and imaging techniques. It can provide quantitative molecular information for an objective diagnosis. Raman spectroscopy can measure both chemical and morphological information in samples and provide objective diagnosis for independent tissue samples of new patients. Some specific techniques and applications presented in this chapter which will demonstrate the potential of Raman spectroscopy for medical diagnostics, as well as the versatile interest in healthcare service.