In order to get the intricate biology of living organisms, the researchers
started using the basic unit of life i.e. “Cell” for elucidating the intricate mechanisms.
This led to the basis of cell culture studies, where cells grown in controlled artificial
conditions simulate the conditions prevailing in natural ones, therefore, presumed to act
as those in in vivo conditions. The introduction of cell culture techniques has helped a
lot in understanding the physiological processes like cell signalling, neurobiology, cell
proliferation, pathogenesis of diseases, apoptosis and even more. In the following
chapter, we will discuss the basics of cell culture including the equipments, chemicals
and different types of materials required for cell culture. The techniques used for
maintenance, preservation and authentication of cell lines are also included.
Keywords: Antibiotics, Aseptic, Cell Culture, Cell Lines, Cryopreservation,
Dimethyl-sulphoxide, Dulbecco’s Minimal Essential Media, Fetal Bovine Serum,
Fibroblast, Glutamine, Hood, Incubator, Media, Phase Contrast Microscope,
Trypsin.
