Fluorescence Microscopy in Life Sciences


Author(s): Juan Carlos Stockert and Alfonso Blazquez-Castro

Pp: 436-461 (26)

DOI: 10.2174/9781681085180117010017

* (Excluding Mailing and Handling)


At present, several histochemical methods for proteins using fluorescent dyes and reagents are available. Some of them utilize simple fluorochromes, whereas others involve preferential fluorogenic reactions with amino acids. Ionic, hydrophobic, and covalent interactions occur between protein groups and fluorochromes. In addition to these classical demonstrations of proteins, more selective methods such as immunofluorescence and detection of enzymatic activity are now widely used in microscopical studies. Also other macromolecules such as nucleic acids, polysaccharides, etc., can be visualized by these methods. Detection of macromolecules is also possible with haptens (i.e. dinitrophenol, biotin, digoxigenin) that are recognizable by suitable ligands (antibodies, streptavidin), or using labeled lectins, toxins, oligonucleotides, etc. (see Chapter 4). Fluorescent reactions for enzymatic activity and immunofluorescence are described in Chapters 14 and 17.

Keywords: Amino groups, Amyloid deposits, Aromatic amino acids, Carboxyl groups, Catecholamines, Dansyl chloride, Fast blue B, Fast red TR, Furanones, Indolamines, Isatin, Isoelectric point, Mercury orange, NBD, Phenanthrenequinone, Sulfhydryls.

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