Trends in Fisheries and Aquatic Animal Health

Fish Histopathology as Biomarker in Ecotoxicology

Author(s): Bozidar Raskovic and Vesna Poleksic

Pp: 155-181 (27)

Doi: 10.2174/9781681085807117010010

* (Excluding Mailing and Handling)


Fish are regarded as valuable organisms in monitoring aquatic ecosystems. They are established as indicator organisms and widely used in both laboratory studies and ecotoxicology. A range of biomarkers are developed for monitoring effects of xenobiotics on fish. Histopathology is classified as an important biomarker in terms of toxicological significance and highly relevant when chronic or sublethal pollutants are present in the water. In this chapter, a short review of histopathological methods is given, with basic guidelines for conducting field studies. The main focus is on different methods for quantifying alterations, namely: semi-quantitative scoring system, histomorphometry and stereology. Advantages and shortcomings of each method are briefly discussed.

Keywords: Ecotoxicology, Fish, Histomorphometry, Quantification, Semiquantitative scoring system, Stereology.

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