Title:Effects of β-glucan and Folium mori Extract Combinations in STZ-induced Diabetic Rats: Effectiveness of Various BGFM Complex Compositions in Treating Diabetes
Volume: 14
Issue: 3
Author(s): Jae-Hak Sohn, Joo Wan Kim, Go-Woon Jung, Dong-Chan Park, Seung-Bae Moon, Hyung-Rae Cho, Sae Kwang Ku*Jae-Suk Choi*
Affiliation:
- Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, 290 Yugok-dong, Gyeongsan-si, Gyeongsanbuk-do, 38610,Korea
- Division of Bio Industry, College of Medical and Life Sciences, Silla University, 140 Baegyang-daero, 700 Beon-gil, Sasang-gu, Busan, 46958,Korea
Keywords:
Composition, diabetes, diabetic complication, Folium mori, streptozotocin, β-glucan.
Abstract: Background: The hypoglycaemic effects of β-glucan (BG) extracts from some mushrooms
and Folium mori (FM) have been reported.
Objective: To determine the most effective composition of combined β-glucan originated from
Aureobasidium and Folium mori extract (BGFM) for treating diabetes and complicated hepatopathy
and nephropathy using streptozotocin (STZ)-induced diabetic rats.
Method: Six days after STZ dosing, rats (n = 5 per group) were selected based on body weight and
blood glucose levels. BGFM was orally administered once a day for 28 days (Qd × 28), and changes
in blood glucose levels, body weight, liver and kidney weight, and levels of serum Blood Urea Nitrogen
(BUN), creatinine, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were
measured.
Results: The diabetic control group exhibited dramatic decreases in body weight, and increase in liver
and kidney weight, and blood glucose, serum BUN, creatinine, AST, and ALT levels compared to the
non-diabetic sham group. However, these hyperglycaemic changes were markedly reduced in the Folium
mori extract single group (S-FM) and some groups that received a mixed composition. Additionally,
the changes related to the complicated hepatopathy and nephropathy of body weight were attenuated
in the β-glucan single (S-BG) and some mixed groups compared to the diabetic control group.
The anti-diabetic effects of β-glucan composition increased with its dose, and similarly, the hypoglycaemic
effects of FM composition increased with its dose. Of the 11 types of mixed composition and
single formulations, the BG:FM 1:4 composition was considered most effective for dual efficacy in
terms of its hypoglycaemic and anti-diabetic effects.
Conclusion: The greatest synergistic effects of the BGFM complex in treating the hyperglycaemic
and complicated hepatopathy and nephropathy of STZ were obtained using a BG:FM 1:4 ratio.
