Title:Formulation and Assessment of In Vivo Anti-Inflammatory Potential of Omega-3-Fatty Acid Loaded Self Emulsifying Nanoemulsion
Volume: 7
Issue: 1
Author(s): Debashree Das, Prashant Sahu, Varsha Kashaw and Sushil K. Kashaw
Affiliation:
Keywords:
Omega-3-fatty acids, anti-inflammatory, papaya latex, oedema, preformulation studies,
nanoemulsion.
Abstract: Background: Formulating omega-3-fatty acids as nanoemulsion solves the issues
associated with conventional anti-inflammatory formulation by increasing solubilization
of the lipophilic moiety, increasing rate of absorption and consequently increasing drug
bioavailability. Thus the principal rationale governing the current analysis was to inspect
the anti-inflammatory potential of nautical omega-3-fatty acids administered orally as
nanoemulsion formulation.
Method: Omega-3-fatty acid concentrate was extracted from cod liver oil by urea crystallization
method and was further exposed to the various physiochemical evaluation parameters
like solubility, partition co-efficient analysis, phytochemical tests and Fourier Transfer
Infra-red spectroscopy (FT-IR). The bioactive concentrate was further encapsulated into
nanoemulsion formulation by means of phase inversion temperature method and characterized
by Scanning electron microscopy (SEM), Particle size and texture evaluations. Wistar
albino rats of either sex were selected and divided into prescribed groups and treated with
omega-3-fatty acid concentrate, omega-3-nanoemulsion and standard drug Diclofenac sodium
respectively and subjected to papaya latex induced rat paw oedema.
Result: The percentage difference in the weight of the oedema induced paw for all treatment
groups were evaluated and the data obtained was compared. All the data were subjected
to due statistical evaluation. The omega-3-nanoemulsion treatment led to a significant
(P<0.05) suppression of paw and ear oedema, in carrageen induced paw oedema, croton
oil induced oedema respectively. Also the extent of oedema suppression was comparatively
more than the suppression observed in case of standard (Diclofenac sodium) treated
group and omega-3-fatty acid concentrate treated group which advocate that omega-3-
nanoemulsion formulation demonstrates potent anti-inflammatory and analgesic efficacy.
