Title:Mechanism and Anticancer Activity of the Metabolites of an Endophytic Fungi from Eucommia ulmoides Oliv
Volume: 17
Issue: 7
Author(s): Qi Li, Yan Zhang, Jun-Ling Shi*, Yi-Lin Wang, Hao-Bin Zhao, Dong-yan Shao, Qing-Sheng Huang, Hui Yang and Ming-Liang Jin
Affiliation:
- Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's,China
Keywords:
Apoptosis, caspase, inhibition effects, pinoresinol, pinoresinol monoglucosid.
Abstract: Backgroud: Pinoresinol (Pin) and pinoresinol monoglucoside (PMG) are plant-derived lignan
molecules with multiple functions. We showed previously that an endophytic fungus from Eucommia ulmoides
Oliv., Phomopsis sp. XP-8 is able to produce Pin and PMG.
Objective: This study was carried out to test the anti-tumor capability of the culture of XP-8 and identify the
major effective compounds.
Method: The fungal culture was added in the culture of HepG2 and K562 cells, and the viabilities of these cells
were detected and the possible mechanism was analyzed.
Result: The fungal culture showed significant capaiblity in decreasing the viability of tumor cells and induce
apoptosis via up-regulation of the expression of apoptosis-related genes. It also significantly inhibited the
adhesion and migration of HepG2 cells by blocking MMP-9 expression. Pin and PMG were isolated from the
growth culture and shown to be the major effective components for inhibition. Conclusion: The study indicated
the potential application of XP-8 in the production of anti-tumour products by the bioconversion of glucose.
