Title:Synthesis and Evaluation of A New Series of Thiazole Derivatives as Potential Antitumor Agents and MMP Inhibitors
Volume: 17
Issue: 5
Author(s): Zafer Asim Kaplancikli, Mehlika Dilek Altıntop*, Ozlem Atli, Belgin Sever, Merve Baysal, Halide Edip Temel, Fatih Demirci and Ahmet Ozdemir
Affiliation:
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir,Turkey
Keywords:
Thiazole, triazole, oxadiazole, anticancer activity, matrix metalloproteinase.
Abstract: Background: In recent years, the relationship between overexpression of matrix metalloproteinases (MMPs)
and tumor invasion/metastasis has prompted researchers to develop MMP inhibitors as anticancer drugs.
Objective: The aim of this study was to design and synthesize new thiazole-based anticancer agents targeting MMPs.
Method: New thiazole derivatives were synthesized and investigated for their cytotoxic effects on A549 human lung
adenocarcinoma, MCF-7 human breast adenocarcinoma and NIH/3T3 mouse embryonic fibroblast cell lines using
MTT assay. The potential inhibitory effects of the best candidates on gelatinases (MMP-2, MMP-9), and collagenases
(MMP-1, MMP-8, MMP-13) were evaluated.
Results: Ethyl 2-[2-((4-amino-5-(phenoxymethyl)-4H-1,2,4-triazol-3-yl)thio)acetamido]-4-methylthiazole-5-carboxylate
(3) was found to be the most promising anticancer agent against MCF-7 cell line due to its selective inhibitory effect
on MCF-7 cells with an IC50 value of 20.6±0.3 μg/mL when compared with cisplatin (IC50= 35.31±0.51 μg/mL).
Compound 3 also showed multiple MMP (MMP-1, MMP-8 and MMP-9) inhibitory activity (10.56±1.70, 20 and
7.28±1.49%, respectively).
Conclusion: The notable anticancer activity and selectivity of compound 3 on MCF-7 cell line can be attributed to
multiple MMP inhibition potential.
