Title:Oleic Acid Protects Against Oxidative Stress Exacerbated by Cytarabine and Doxorubicin in Rat Brain
Volume: 16
Issue: 11
Author(s): David Calderón Guzmán, Norma Osnaya Brizuela, Maribel Ortíz Herrera, Hugo Juárez Olguín, Ernestina Hernández García, Armando Valenzuela Peraza and Gerardo Barragán Mejía
Affiliation:
Keywords:
Brain, cytarabine, doxorubicin, oxidative damage, unsaturated fatty acid.
Abstract: Aim: The objective of this study was to analyze the effect of doxorubicin and
cytarabine on biogenic amines and oxidative biomarkers in the brain of rats treated with oleic
acid.
Methods: Thirty-six Wistar rats distributed in 6 groups, were treated as follows: group 1
(control), NaCl 0.9%; group 2 doxorubicin (1mg/kg); group 3 cytarabine (70mg /kg); group 4
oleic acid (1500μl/kg); group 5 doxorubicin + oleic acid; group 6 cytarabine + oleic acid. All
compounds were administered intraperitoneally for 5 days. The Rats were sacrificed after
receiving the last administration and their brains were dissected in cortex, hemispheres, and
cerebellum/medulla oblongata. Blood samples were obtained on sacrifice to assess the levels of
glucose and triglycerides. In each brain region, lipoperoxidation (TBARS), H2O2, Na+, K+
ATPase, glutathione (GSH), serotonin metabolites (5-HIAA) and dopamine were measured
using validated methods.
Results: Cytarabine decreased the levels of dopamine, TBARS, GSH, H2O2 and ATPase in all regions. Doxorubicin
combined with oleic acid increased the levels of GSH in cortex, and decreased ATPase in cerebellum/medulla
oblongata.
Conclusion: These results suggest that the reduction of dopamine and oxidant effect during cytarabine treatment could
result in brain injury but could be prevented by oleic acid supplementation.
