Title:Alterations of the X Chromosome in Lymphocytes of Alzheimer’s Disease Patients
Volume: 12
Issue: 10
Author(s): Biljana Spremo-Potparevic, Vladan Bajic, George Perry and Lada Zivkovic
Affiliation:
Keywords:
Alzheimer’s disease, aneuploidy, FISH, peripheral blood lymphocytes, PCD, X chromosome.
Abstract: Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer’s disease
(AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a
method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome
alterations in the possible etiology of AD females, we used fluorescent in situ hybridization
(FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature
centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results
showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched
controls (p<0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared
with age matched controls (p<0.001). In addition, a strong (R2=0.97, n=20) and significant (p<0.001) correlation
was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a
generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.
