Title:Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells
Volume: 10
Issue: 2
Author(s): Xiao-Yue Deng, Hu Wang, Tao Wang, Xian-Tao Fang, Li-Li Zou, Zhi-Ying Li and Chang-Bai Liu
Affiliation:
Keywords:
DNA integration-free, induced pluripotent stem cells, non-viral methods.
Abstract: Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression
of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential
increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At
the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of
viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral
methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction
of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these
non-viral methods.
