Title:The Synthesis and Evaluation of Novel Lactate Dehydrogenase Nanobioconjugates
Volume: 8
Issue: 2
Author(s): Samina Nazir, Tajammul Hussain, Salman Akbar Malik and Ayesha Younus
Affiliation:
Keywords:
Nanobioconjugates, Metal nanoparticles, LDH-Ni conjugate, LDH- Fe3O4 conjugate, Enzyme affinity, Km, Enzyme kinetics,
Lactate dehydrogenase, cancer
Abstract: We, report for the first time, the synthesis and kinetics of Ni and Fe3O4 nanobioconjugates obtained by covalent conjugation of
nanoparticles to lactate dehydrogenase (LDH). The conjugation reaction was performed using bis(sulfosuccinimidyl) suberate (BS3) as a
cross-linking agent. The kinetics of discrete conjugates was studied through conversion of lactate and pyruvate and subsequent reduction
of NAD+ to NADH at 340 nm. The enzyme velocities and the Km values designated that LDH-Ni conjugate developed a higher affinity
for substrate. The fabrication of LDH-Ni conjugates makes them promising candidates for biotechnology applications. These novel findings
pave way to device a convenient nanoparticle-based LDH assay void of any radioactive isotope or use of complicated dyes for invitro
detection and management of malignancies such as leukemia, malaria, to determine cytotoxic potential of compounds in food, cosmetics
and pharmaceutical industries. Furthermore, the altered comportment of novel conjugates may serve as a guideline in the development
of nanobioconjugates and new advances in nanoparticle based diagnosis and therapeutics.