Title:Effect of 3',4'-dihydroxy Flavonol Supplementation for One Week on Renal Functions and Lipid Peroxidation as Distant Organ Damage After Brain Ischemia-reperfusion in Rats
Volume: 22
Issue: 3
Author(s): Merve Gulen, Tugce Aladag, Gozde Acar, Abdulkerim Kasim Baltaci and Rasim Mogulkoc*
Affiliation:
- Department of Physiology, Medical School, Selçuk University, Konya, Turkiye
Keywords:
3', 4'-dihydroxyflavonol, brain ischemia reperfusion, kidney injury, glutathione, malondialdehyde, elements.
Abstract:
Introduction: The objective of this study was to investigate the effects of 3',4'-
dihydroxyflavonol (DiOHF) on oxidative and antioxidant systems in kidney tissue and on renal
function as distant organ damage following brain ischemia-reperfusion.
Methods: This study was conducted on 28 male Wistar-Albino rats, which were divided into
four groups: Control, Sham, Ischemia-Reperfusion (I/R), and Ischemia-Reperfusion + DiOHF.
Kidney tissue samples were collected to analyze malondialdehyde (MDA) and glutathione
(GSH) levels. Additionally, concentrations of electrolytes (Ca, Cl, Na, K, and P), as well as urea,
uric acid, creatinine, and urinary microprotein levels, were measured.
Results: Brain ischemia-reperfusion led to increased malondialdehyde (MDA) levels in both the
kidney medulla and cortex, indicating oxidative stress in these distant organs, while glutathione
(GSH) levels were suppressed. Additionally, ischemia-reperfusion caused elevations in blood
and urine concentrations of urea, uric acid, creatinine, and urinary microproteins.
Discussion: This experimental model significantly elevated urea, uric acid, and creatinine levels,
key indicators of kidney function, and similarly increased urinary microprotein loss. While our
study demonstrates the detrimental effects of focal brain ischemia-reperfusion on kidney function
as a distant organ, further research is needed to investigate its impact on other organs to gain
a more comprehensive understanding of distant organ damage.
Conclusion: Results from this experimental model indicate that cerebral ischemia-reperfusion in
rats suppresses the antioxidant system and increases oxidative stress in kidney tissue, leading to
impaired renal function. However, a 1-week DiOHF treatment mitigated this damage by enhancing
the antioxidant defense system.
