Title:Green Synthesis of Silver Nanoparticles using Punica granatum Leaf Extract: A Novel Approach to Combat Quinolone-Resistant Urinary Tract Infective Pathogens
Volume: 21
Issue: 1
Author(s): Md. Abdullah Al Mashud, Md. Moinuzzaman, Masuma Anzuman, Nasrin Islam Moon, Shovon Shaha, Md. Helal Uddin, Rizone Al Hasib*, Nilufa Akhter Banu, Ajoy Kumer*Mohammad Abu Hena Mostofa Jamal*
Affiliation:
- Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh
- Laboratory of Medical and Environmental Biotechnology, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh
- Department of Chemistry, College of Arts
and Sciences, IUBAT-International University of Business Agriculture and Technology, 4-Embankment Drive
Road, Sector 10, Uttara Model Town, Dhaka, 1230, Bangladesh
- Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh
- Laboratory of Medical and Environmental Biotechnology, Faculty of Biological Sciences, Islamic University, Kushtia, 7003, Bangladesh
Keywords:
Nanoparticles, urinary tract infections, quinolones, antibacterial agents, drug-resistance, Punica granatum.
Abstract:
Introduction: Nanoparticles obtained through green synthesis play remarkable
roles in biomedical applications. Urinary tract infections (UTIs) are a nightmare for the
mass population, especially for women, and quinolone-resistant UTI bacteria worsen the
situation. Our current investigation aimed to control quinolone-resistant pathogenic UTI
bacteria with green-synthesized silver nanoparticles (AgNPs).
Methods: Visual observation of color change, UV-Vis spectroscopic analysis, FTIR (Fourier
Transform Infrared Spectroscopy), DLS (Dynamic Light Scattering), XRD (X-ray Diffraction),
and TEM (Transmission Electron Microscopy) techniques were used to effectively
characterize the biosynthesized AgNPs. Klebsiella variicola, Pseudomonas sp., and
Staphylococcus epidermidis bacteria were isolated and identified using biochemical and
molecular identification techniques from urine samples of hospitalized patients with UTI.
These bacteria showed quinolone resistance to up to fourth-generation antibiotics.
Results and Discussion: The results elucidated the synthesis of spherical-shaped nanosilvers
coated with Punica granatum polyphenols. These biosynthesized AgNPs showed
moderate polydispersity and narrow distribution. The antibacterial efficiency of the AgNPs
was determined against isolated bacterial strains. Klebsiella variicola and Staphylococcus
epidermidis exhibited the highest sensitivity to the nanoparticles. Nanoparticles at a concentration
of 128 μg/ml inhibited bacterial growth to a great extent and gave a maximum
inhibition zone of 14.67 ± 0.577 mm in diameter for both bacterial strains. In addition, toxicity
analysis of synthesized nanoparticles via brine shrimp lethality assay (BSLA) showed
a very low cytotoxicity level (2398.83 μg/ml), depicting safety for human use.
Conclusion: We can conclude that Punica granatum leaf-synthesized AgNPs could possess
significant biomedical applications as potential antibacterial agents due to their bactericidal
activity and low cytotoxicity.
