Title:Hesperidin Improves Wound Healing and Mineralization of Periodontal Ligament Cells in Elevated Glucose Conditions
Volume: 32
Issue: 33
Author(s): Smriti Aryal A.C.*, Md Sofiqul Islam, Aghila Rani K.G., Mohannad Nassar and Mohammed Mustahsen Rahman
Affiliation:
- Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, 27272, United Arab Emirates
Keywords:
Diabetic culture, glucose, hesperidin, HPN, human periodontal ligament fibroblast cells, mineralization, wound healing.
Abstract:
Introduction: Elevated glucose can have a detrimental effect on the function
and healing process of periodontal cells in inflammatory conditions. Hesperidin (HPN),
a bioflavonoid found abundantly in citrus fruits, has numerous biological benefits, including
regenerative and anti-inflammatory properties. The current in vitro study aimed
to assess the impact of HPN on the proliferation, wound healing, and functionality of periodontal
cells in optimal and elevated glucose conditions.
Methods: Human periodontal ligament cells (HPDLCs) were cultured in optimal glucose
(1g/L) (OG) and high glucose (4.5 g/L) (HG) conditions. XTT, wound healing,
ALP, and calcium release assays were conducted with or without HPN in the culture media.
Results: The statistical analysis revealed that adding different concentrations of HPN (2,
4, 10, or 100 μM) had no significant effect on the viability of HPDLCs under both OG
(p=0.436) and HG conditions (p=0.162) compared to the control. However, in the HG
condition, the addition of 100 μM HPN resulted in a statistically significant increase in
wound closure (p=0.003). Furthermore, in the HG condition, the addition of 100 μM
HPN significantly increased ALP activity in the non-osteogenic media (p=0.001) and
significantly increased calcium release within the osteogenic media (p=0.016).
Conclusion: The findings of this study suggest that HPN provides beneficial effects, facilitating
repair and mineralization in HPDLCs under HG conditions.
