Title:Effects of Sodium Hypochlorite and Chlorine Dioxide on Human Root Canal Dentine: An ATR-FTIR Spectroscopy Study
Volume: 20
Issue: 9
Author(s): Hilal Erdogan*, Funda Kont Cobankara and Mustafa Topkafa
Affiliation:
- Department of Endodontics, Faculty of Dentistry, Nevsehir Haci Bektas Veli University, Nevsehir, Turkey
Keywords:
Dental materials, chlorine dioxide, demineralization, fourier transform infrared spectroscopy, protein denaturation, surface properties.
Abstract:
Background: It is very important to assess the effects of NaOCl and ClO2 on dentine
deproteination because these solutions are in contact with dentine during endodontic treatment
and may affect the physical and chemical structure of dentine.
Objectives: This study aimed to analyze the effects of sodium-hypochlorite (NaOCl) and chlorinedioxide (ClO2) on the chemical structure of human dentine by Attenuated Total Reflection Fourier
Transform Infrared (ATR-FTIR) spectroscopy.
Methods: Fifteen human maxillary incisor roots were cut longitudinally into two parallel dentin
discs being approximately 4x4x2 mm in size. 30 samples were randomly allocated to 6 groups
treated with 5.25% NaOCl or 0.014% ClO2 (1, 5, or 10 minutes); self-control was used. The effect
of solutions on the organic and inorganic components of the radicular dentine surface was analyzed
using the amide:phosphate ratio and carbonate:phosphate ratio. The intragroup differences
(paired t-test) and intergroup differences (one-way analysis of variance and Tukey’s posthoc test)
were analyzed.
Results: The intragroup comparisons showed the amide:phosphate ratio to be higher at all times at
ClO2. NaOCl caused a decrease in the amide:phosphate ratio at 10 minutes (p<0.05). The intergroup
comparison showed that NaOCl caused a greater decrease in amide:phosphate at all times
compared to ClO2 (p<0.05). All comparisons demonstrated no significant difference in the carbonate:
phosphate ratio (p˃0.05).
Conclusion: Considering the results of this study, it is recommended to avoid prolonged exposure
to minimize NaOCl-induced dentine deproteination. It should also be taken into account that ClO2
increases the amide:phosphate ratio in radicular dentine, and this effect is advantageous in clinical
use for collagen structure, contrary to the negative impact of NaOCl.