Title:Polymeric Vehicles for Nucleic Acid Delivery: Enhancing the Therapeutic Efficacy and Cellular Uptake
Volume: 18
Issue: 4
Author(s): Parul Gupta, Anjali Sharma*Vishnu Mittal
Affiliation:
- Department of Pharmaceutics, Guru Gobind Singh College of Pharmacy, Yamunanagar, Haryana, 135001, India
Keywords:
Nucleic acid, polymer, immunity, biodegradable, biocompatible, polymeric vehicle.
Abstract:
Background: Therapeutic gene delivery may be facilitated by the use of polymeric carriers.
When combined with nucleic acids to form nanoparticles or polyplexes, a variety of polymers
may shield the cargo from in vivo breakdown and clearance while also making it easier for it to
enter intracellular compartments.
Aim and Objectives: Polymer synthesis design choices result in a wide variety of compounds and
vehicle compositions. Depending on the application, these characteristics may be changed to provide
enhanced endosomal escape, longer-lasting distribution, or stronger connection with nucleic
acid cargo and cells. Here, we outline current methods for delivering genes in preclinical and clinical
settings using polymers.
Methodology: Significant therapeutic outcomes have previously been attained using genetic material-
delivering polymer vehicles in both in-vitro and animal models. When combined with nucleic acids
to form nanoparticles or polyplexes, a variety of polymers may shield the cargo from in vivo
breakdown and clearance while also making it easier for it to enter intracellular compartments.
Many innovative diagnoses for nucleic acids have been investigated and put through clinical
assessment in the past 20 years.
Results: Polymer-based carriers have additional delivery issues due to their changes in method and
place of biological action, as well as variances in biophysical characteristics. We cover recent custom
polymeric carrier architectures that were tuned for nucleic acid payloads such genomemodifying
nucleic acids, siRNA, microRNA, and plasmid DNA.
Conclusion: In conclusion, the development of polymeric carriers for gene delivery holds promise
for therapeutic applications. Through careful design and optimization, these carriers can overcome
various challenges associated with nucleic acid delivery, offering new avenues for treating a wide
range of diseases.
