Title:Cytotoxic Impact of Naringenin-Loaded Solid Lipid Nanoparticles on RIN5F Pancreatic β Cells via Autophagy Blockage
Volume: 18
Issue: 4
Author(s): Pardis Mohammadi Pour, Zeinab Nouri, Dariush Ghasemi, Soraya Sajadimajd and Mohammad Hosein Farzaei*
Affiliation:
- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical
Sciences, Kermanshah, Iran
Keywords:
Autophagy, pancreatic cancer, miRNA, naringenin-loaded solid lipid nanoparticle, Rin-5F cells, DNA mutations.
Abstract:
Background: Autophagy plays a crucial role in modulating the proliferation of cancer
diseases. However, the application of Naringenin (Nar), a compound with potential benefits against
these diseases, has been limited due to its poor solubility and bioavailability.
Objective: This study aimed to develop solid lipid nanoparticles (Nar-SLNs) loaded with Nar to enhance
their therapeutic impact.
Methods: In vitro experiments using Rin-5F cells exposed to Nar and Nar-SLNs were carried out to
investigate the protective effects of Nar and its nanoformulation against the pancreatic cancer cell
line of Rin-5F.
Results: Treatment with Nar and Nar-SLN led to an increase in autophagic markers (Akt, LC3, Beclin1,
and ATG genes) and a decrease in the level of miR-21. Both Nar and Nar-SLN treatments inhibited
cell proliferation and reduced the expression of autophagic markers. Notably, Nar-SLNs exhibited
greater efficacy compared to free Nar.
Conclusion: These findings suggest that SLNs effectively enhance the cytotoxic impact of Nar,
making Nar-SLNs a promising candidate for suppressing or preventing Rin-5F cell growth.
