Title:Spectrophotometric Determination of Curcumin using 3-Methyl-2-benzothiazolinone Hydrazone Hydrochloride Hydrate as Electrophilic Coupling Agent
Volume: 14
Issue: 3
Author(s): Syeda Ayesha*
Affiliation:
- Department of Chemistry, GFGC, Kuvempunagar, Mysore, 570023, Karnataka, India
Keywords:
Nutraceutical, turmeric, curcumin, curcuma longa, spectrophotometry, MBTH.
Abstract:
Introduction: Nutraceuticals are products derived from food sources that provide
extra health benefits in addition to the basic nutritional value found in foods. Spectrophotometric
methods for the determination of curcumin were developed, which are
simple, sensitive, selective, rapid, and reliable.
Methods: The methods are based on the reduction of iron (III) to iron (II) by 3-Methyl-2-
benzothiazolinone hydrazone hydrochloride hydrate (MBTH), which is an electrophilic
coupling agent and subsequent reaction with curcumin in mild hydrochloric acid medium.
Spectrophotometric methods for the determination of curcumin were developed, which
are simple, sensitive, selective, rapid, and reliable. The methods are based on the reduction
of iron (III) to iron (II) by 3-Methyl-2-benzothiazolinone hydrazone hydrochloride
hydrate (MBTH), which is an electrophilic coupling agent and subsequent reaction with
curcumin in mild hydrochloric acid medium.
Result: The reaction produces a bluish–green complex with maximum absorbance at 670
nm. The colour complex can be extracted into chloroform. The methods obey Beer’s law
in the range 0.3-7.0 and 0.2-7.0 (μg mL-1).
Conclusion: 10 common anions and cations were added, and the method was tested, and
no interference was observed. The proposed methods offered the advantages of having
good reproducibility and were satisfactorily worked out to estimate the amount of curcumin
in various turmeric samples.
