Title:Applicability of Salting-out Liquid-liquid Extraction for New Fluorimetric Quantification of Omadacycline in Pharmaceutical Formulations and Biological Samples
Volume: 20
Issue: 5
Author(s): Eman Alzahrani, Mohamed A. Abdel-Lateef*Baher I. Salman*
Affiliation:
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut
71524, Egypt
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut
71524, Egypt
Keywords:
OMC, 4-chloro-7-nitrobenzofurazan, human plasma analysis, fluorimetry, pharmaceutical analysis, milk analysis.
Abstract:
Objectives: A detectable innovative fluorimetric method was used to determine omadacycline
(OMC) in human plasma matrices, pharmaceutical tablets, and vials with high recovery
rates and without biological interference.
Methods: The fluorimetric technique was used based on the interaction between 4-chloro7-nitrobenzofurazan
(NBD-Cl) with a (2-ry amine group) in OMC at pH 8.0, which generates a fluorescent
compound measured at 530 nm (exci 470 nm) following a 10-minute heating step at 80 oC.
The plasma and milk samples were treated with ammonium sulfate as a salting-out procedure.
Results: Omadacycline (OMC) was successfully determined in pharmaceuticals, plasma, and milk
samples with a linear range from 60.0 to 700.0 ng mL-1, with the lower limit of detection (LOD
5.18 ng mL-1) and limit of quantitation (LOQ 15.72 ng mL-1).
Conclusion: This simple, reliable, and detectable fluorimetric method was successfully developed
to determine omadacycline in pharmaceutical tablets, plasma samples, and milk with high recovery
rates.
