Title:Green Synthesis of Silver Nanoparticles using Cirsium congestum Extract
Modified by Chitosan/Alginate: Bactericidal Activity against Pathogenic
Bacteria and Cytotoxicity Analysis in Normal Cell Line
Volume: 30
Issue: 20
Author(s): Mahnaz Mohtashami, Alieh Rezagholizade-Shirvan, Zahra Hojati Bonab, Mohammad Reza Amiryousefi*, Majid Darroudi, Mobina Sadat Ahmadi Solimani, Sajad Yaghoobi, Samaneh Dolatabadi, Ahmad Ghasemi and Amir Abbas Momtazi-Borojeni*
Affiliation:
- Department
of Food Science and Technology, Neyshabur University of Medical Sciences, Neyshabur, Iran
- Healthy Ageing Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran
- Department
of Medical Biotechnology, School of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran
Keywords:
Green synthesis, chitosan, alginate, antibacterial activity, cytotoxicity, cell apoptosis.
Abstract:
Aim: The study aimed to determine in vitro pharmacological effects of modified Ag nanoparticles
(AgNPs).
Background: AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized
AgNPs (cAgNPs) has raised challenges that limit their use.
Objective: The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized
using Cirsium congestum extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs).
Methods: Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of
Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of
Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and
apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.
Results: Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed,
and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in
a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs.
The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity
effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results
showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to
when the cell was treated with cAgNPs.
Conclusion: Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.
